::Affinity Photoprobes, LLC::

	8-Azidoguanosine 5'-[a32P]triphosphate ([a32P]8N3GTP)
	8-Azidoguanosine 5'-[a32P]diphosphate ([a32P]8N3GDP)
	8-Azidoguanosine 5'-[g32P]triphosphate ([g32P]8N3GTP)
	Guanosine 5'-[g32P]triphosphate [g] 4-Azidoanilide ([g32P]GTPgAA)
	Guanosine 5'-[a32P] triphosphate [g] 4-Azidoanilide ([a32P]GTPgAA)

8-Azidoguanosine 5'-[a32P]triphosphate ([a32P]8N3GTP)

       For Prices See Product Order No. AP04

Molecular Formula: C₁₀H₁₅N₈0₁₄P₃ for the free acid

Molecular Weight: 564.2 for the free acid

Description: Triethylammonium salt solution in absolute methanol

UV Characteristics:

Absorbance maximum (l_max) = 278 nm at pH 7.0

Molar extinction coefficient (e ) 12000

Photoactivity confirmed by recording UV spectra before and after photolysis with hand-held UV lamp.

Assay: Approximately 95% by UV spectral determination

Storage: Store at -20^oC, protect from light.  Compound is not affected by ordinary room lighting including fluorescent light.

 

8-Azidoguanosine 5'-[a32P]diphosphate ([a32P]8N3GDP)

For Prices See Product Order No. AP09

Molecular Formula: C₁₀H₁₅N₈0₁₁P₂ for the free acid

Molecular Weight: 485.2 for the free acid

Description: Triethylammonium salt solution in absolute methanol

UV Characteristics:

Absorbance maximum (l_max) = 278 nm at pH 7

Molar extinction coefficient (e ) 12000

Photoactivity confirmed by recording UV spectra before and after photolysis with hand-held UV lamp.

Assay: Approximately 95% by UV spectral determination

Storage: Store at -20^oC, protect from light.  Compound is not affected by ordinary room lighting including fluorescent light.

 

8-Azidoguanosine 5'-[g32P]triphosphate ([g32P]8N3GTP)

       For Prices See Product Order No. AP05

Specific activity: 10-15 Ci/mmol: 370-555 GBq/mmol; 1-4 mCi/ml; 37-148 MBq/ml

Molecular Formula: C₁₀H₁₅N₈0₁₄P₃ for the free acid

Molecular Weight: 564.2 for the free acid

Description: Triethylammonium salt solution in absolute methanol

UV Characteristics:

Absorbance maximum (l_max) = 278 nm at pH 7.0

Molar extinction coefficient (e ) =12000

Photoactivity confirmed by recording UV spectra before and after photolysis with hand-held UV lamp.

Assay: Approximately 95% by UV spectral determination

Storage: Store at -20^oC in 100% methanol,  protect from UV light and sunlight.   Compound is not affected by ordinary room lighting including fluorescent light.

 

Guanosine 5'-[g32P]triphosphate [g] 4-Azidoanilide ([g32P]GTPgAA)

 For Pricing See Product Order No. GP01 and GPH01 (High specific activity)

Specific activity:

GP01: 10-15 Ci/mmol: 370-555 GBq/mmol; 1-4 mCi/ml, 37-148 MBq/ml

GPH01: 60-80 Ci/mmol: 2200-2960 GBq/mmol; 1-4 mCi/ml, 37-148 MBq/ml

Molecular Formula: C₁₆H₂₀N₉0₁₃P₃ for the free acid

Molecular Weight: 639.3 for the free acid

Description: Triethylammonium salt solution in absolute methanol

UV Characteristics:

Absorbance maximum (l_max) = 257 nm at pH 7.0

Molar extinction coefficient (e ) = 30600

Photoactivity confirmed by recording UV spectra before and after photolysis with hand-held UV lamp.

Assay: Approximately 90% by thin layer and UV spectral analysis.

Storage: Store at -20^oC in 100% methanol, protect from UV light including sunlight.   Compound is not affected by ordinary room lighting including fluorescent light.

 

Guanosine 5'-[a32P] triphosphate [g] 4-Azidoanilide ([a32P]GTPgAA)

For Prices See Product No. AGP01

Specific activity:  10-15 Ci/mmol: 370-555 GBq/mmol; 1-4 mCi/ml, 37-148 MBq/ml

Molecular Formula: C₁₆H₂₀N₉0₁₃P₃ for the free acid

Molecular Weight: 639.3 for the free acid

Description: Triethylammonium salt solution in absolute methanol

UV Characteristics:

Absorbance maximum (l_max) = 257 nm at pH 7.0

Molar extinction coefficient (e ) = 30600

Photoactivity confirmed by recording UV spectra before and after photolysis with hand-held UV lamp.

Assay: Approximately 90% by thin layer and UV spectral analysis.

Storage: Store at -20^oC in 100% methanol, protect from UV light including sunlight.   Compound is not affected by ordinary room lighting including fluorescent light.

 

[g³²P]GTPgAA is a nonhydrolyzable GTP analog which has a phenyl azide group substituted off the gamma phosphate. [g³²P]GTPgAA has been reported to be useful for the study G-proteins (see G-Protein Methods, ALT Tech Brief 1 and 2511428).  With these two structural photoaffinity isomers of GTP, it is possible to completely map out both the guanine base binding portion as well as the triphosphate binding domain of the active site of virtually any GTP binding protein.   The active site site peptides of a protein can be easily determined using a combination of photoaffinity labeling, proteolytic digestion, immobilized metal affinity chromatography (IMAC) followed by reverse phase HPLC (see 8106359, 8439545). 

References (see Azido-GTP Photoaffinity Analogs)