::Affinity Photoprobes, LLC::

	5-Azidouridine 5'-triphosphate (5N3UTP)
	5-Azidouridine 5'-diphosphate (5N3UDP)
	5-Azidouridine 5'-monophosphate (5N3UMP)
	5-Azidouridine (5N3Uridine)
	5-Azidouridine 5'-diphospho Glucose (5N3UDP-Glu)
	5-Azidouridine 5'-diphospho Glucuronic acid (5N3UDP-GlcA)
	5-Azido 2'-deoxyuridine 5'-triphosphate (5N3dUTP)
	5-Azido 2'-deoxyuridine 5'-diphosphate (5N3dUDP)
	5-Azido 2'-deoxyuridine 5'-monophosphate (5N3dUMP)
	5-Azido 2'-deoxyuridine (5N3dUridine) We also have or can easily make the 5-Amino (5NH2-) analog for all the uridine nucleotide and nucleoside analogs listed above. All 5-Azidouridine photoaffinity analogs are licensed under U.S. Patent No. 4,672,111. These products are for research purposes only.

5-Azidouridine 5'-triphosphate (5N3UTP)

For Prices See Order No. AT03

Molecular Formula: C₉H₁₄N₅0₁₅P₃ for the free acid

Molecular Weight: 525.16 for the free acid

Description: Triethylammonium salt solution in absolute methanol

UV Characteristics:

Absorbance maximum (l_max) = 288 nm at pH 7.0

Molar extinction coefficient (e ) = 7600

Photoactivity confirmed by recording UV spectra before and after photolysis with a hand-held UV lamp.

Assay: >98% by TLC

Storage: Store at -20^oC, protect from UV light and sunlight.  Compound is not affected by ordinary room lighting including fluorescent light.

5-Azidouridine 5'-diphosphate (5N3UDP)

 For Prices See Order No. AT23

Molecular Formula: C₉H₁₄N₅0₁₂P₂ for the free acid

Molecular Weight: 446.16 for the free acid

Description: Triethylammonium salt solution in absolute methanol

UV Characteristics:

Absorbance maximum (l_max)= 288 nm at pH 7.0

Molar extinction coefficient (e ) = 7600

Photoactivity confirmed by recording UV spectra before and after photolysis with a hand-held UV lamp.

Assay: Approximately 95% by thin layer and UV spectral analysis.

Storage: Store at -20^oC, protect from UV light and sunlight.  Compound is not affected by ordinary room lighting including fluorescent light.

5-Azidouridine 5'-monophosphate (5N3UMP)

For Prices See Order No. AT24

Molecular Formula: C₉H₁₄N₅0₉P for the free acid

Molecular Weight: 367.16 for the free acid

Description: Triethylammonium salt solution in absolute methanol

UV Characteristics:

Absorbance maximum (l_max)= 288 nm at pH 7.0

Molar extinction coefficient (e ) = 7600

Photoactivity confirmed by recording UV spectra before and after photolysis with a hand-held UV lamp.

Assay: Approximately 95% by thin layer and UV spectral analysis.

Storage: Store at -20^oC, protect from UV light and sunlight.  Compound is not affected by ordinary room lighting including fluorescent light.

5-Azidouridine (5N3Uridine)

For Prices See Order No. AT25

Molecular Formula: C₉H₁₄N₅0₆ for the free acid

Molecular Weight: 288.16 for the free acid

Description: Triethylammonium salt solution in absolute methanol

UV Characteristics:

Absorbance maximum (l_max)= 288 nm at pH 7.0

Molar extinction coefficient (e ) = 7600

Photoactivity confirmed by recording UV spectra before and after photolysis with a hand-held UV lamp.

Assay: Approximately 95% by thin layer and UV spectral analysis.

Storage: Store at -20^oC, protect from UV light and sunlight.  Compound is not affected by ordinary room lighting including fluorescent light.

5-Azidouridine 5'-diphospho Glucose (5N3UDP-Glu).

For Prices See Order No. AT10

Molecular Formula: C₁₅H₂₃N₅0₁₇P₂ for the free acid

Molecular Weight: 607.3 for the free acid

Description: Triethylammonium salt solution in absolute methanol

UV Characteristics:

Absorbance maximum (l_max)= 288 nm at pH 7.0

Molar extinction coefficient (e ) = 7600

Photoactivity confirmed by recording UV spectra before and after photolysis with a hand-held UV lamp.

Assay: Approximately 95% by thin layer and UV spectral analysis.

Storage: Store at -20^oC, protect from UV light and sunlight.  Compound is not affected by ordinary room lighting including fluorescent light.

5-Azidouridine 5'-diphospho Glucuronic acid (5N3UDP-GlcA)

For prices see Order No. AT19

Molecular Formula: C₁₅H₂₁N₅0₁₈P₂ for the free acid

Molecular Weight: 621.27 for the free acid

Description: Triethylammonium salt solution in absolute methanol

UV Characteristics:

Absorbance maximum (l_max)= 288 nm at pH 7.0

Molar extinction coefficient (e ) = 7600

Photoactivity confirmed by recording UV spectra before and after photolysis with a hand-held UV lamp.

Assay: Approximately 95% by thin layer and UV spectral analysis.

Storage: Store at -20^oC, protect from UV light and sunlight.  Compound is not affected by ordinary room lighting including fluorescent light.

5-Azido-2’-deoxyuridine triphosphate  (5N3dUTP)

 For Prices See Order No. AT14

Molecular Formula: C₉H₁₃N₅0₁₄P₃ for the free acid

Molecular Weight: 508.07 for the free acid

Description: Triethylammonium salt solution in absolute methanol

UV Characteristics:

Absorbance maximum (l_max) = 288 nm at pH 7.0

Molar extinction coefficient (e ) = 7600

Photoactivity confirmed by recording UV spectra before and after photolysis.

Assay: >98% by TLC

Storage: Store at -20^oC, protect from UV light and sunlight.  Compound is not affected by ordinary room lighting including fluorescent light.

5-Azido 2'-deoxyuridine 5'-diphosphate (5N3dUDP)

For prices see Order No. AT26

Molecular Formula: C₉H₁₃N₅0₁₁P₂ for the free acid

Molecular Weight: 429.07 for the free acid

Description: Triethylammonium salt solution in absolute methanol

UV Characteristics:

Absorbance maximum (l_max)= 288 nm at pH 7.0

Molar extinction coefficient (e ) = 7600

Photoactivity confirmed by recording UV spectra before and after photolysis with a hand-held UV lamp.

Assay: Approximately 95% by thin layer and UV spectral analysis.

Storage: Store at -20^oC, protect from UV light and sunlight.  Compound is not affected by ordinary room lighting including fluorescent light.

5-Azido 2'-deoxyuridine 5'-monophosphate (5N3dUMP)

For prices see Order No. AT27

Molecular Formula: C₉H₁₃N₅0₈P for the free acid

Molecular Weight: 350.07 for the free acid

Description: Triethylammonium salt solution in absolute methanol

UV Characteristics:

Absorbance maximum (l_max)= 288 nm at pH 7.0

Molar extinction coefficient (e ) = 7600

Photoactivity confirmed by recording UV spectra before and after photolysis with a hand-held UV lamp.

Assay: Approximately 95% by thin layer and UV spectral analysis.

Storage: Store at -20^oC, protect from UV light and sunlight.  Compound is not affected by ordinary room lighting including fluorescent light.

5-Azido 2'-deoxyuridine (5N3dUridine)

For prices see Order No. AT28

Molecular Formula: C₉H₁₃N₅0₅ for the free acid

Molecular Weight: 271.07 for the free acid

Description: Triethylammonium salt solution in absolute methanol

UV Characteristics:

Absorbance maximum (l_max)= 288 nm at pH 7.0

Molar extinction coefficient (e ) = 7600

Photoactivity confirmed by recording UV spectra before and after photolysis with a hand-held UV lamp.

Assay: Approximately 95% by thin layer and UV spectral analysis.

Storage: Store at -20^oC, protect from UV light and sunlight.  Compound is not affected by ordinary room lighting including fluorescent light.

5N₃dUTP Substitutes for thymidine in the synthesis of photoactive DNA and 5N₃UTP UTP in the synthesis of photoactive RNA (see Photoactive DNA and RNA).  The radioactive label can be attached by using [a³²P]ATP, [a³²P]GTP or [a³²P]CTP.   Additionally 8N₃ATP and 8N₃GTP can be used to produce photoactive RNA.

References (see also Azido-UTP Photoaffinity Analogs)

	Synthesis and biological properties of 5-azido-2'-deoxyuridine 5'-triphosphate, a photoactive nucleotide suitable for making light-sensitive DNA. Evans RK, Haley BE. Biochemistry. 1987 Jan 13; 26(1): 269-76. (3548818)
	5-Azido-2'-deoxyuridine 5'-triphosphate: a photoaffinity-labeling reagent and tool for the enzymatic synthesis of photoactive DNA. Evans RK, Johnson JD, Haley BE.  Proc Natl Acad Sci U S A. 1986 Aug; 83(15): 5382-6. (3461438)